Team:Arizona State/Lab/Protocols/Media

SOB / SOC

"Super optimal broth" (SOB) and "super optimal broth with catabolite repression" (SOC) are nutrient-rich media. Use extreme caution (and if possible make only small amounts) when manipulating, as they are very easy to contaminate.

For 1 L SOB, combine:

• 20 g Tryptone
• 5 g Yeast extract
• 0.5 g NaCl (8.56 mM)
• 1.86 g KCl (2.5 mM)
• ddH₂O to 1 L

Autoclave.

SOC: Add to SOB:

• 0.952 g MgCl2 (10 mM) or 2.408 g MgSO4 (20 mM)
• 3.603 g glucose (20 mM)

Do not autoclave SOC, as the glucose will react with the tryptone. Either autoclave glucose separately or filter sterilize the SOC.

LB agar

• Combine 3.7 g LB agar with 100 ml H₂O. Autoclave or microwave. Makes ~4 plates.

LB broth

• For 1 L, add to 800ml water:

• 10 g tryptone
• 5 g yeast extreact
• 10 g NaCl

• Adjust ph to 7.5
• Adjust volume to 1 L
• Autoclave

Trypticase soy broth

Trypticase soy agar

• For 1 L:

• 30 g soy broth
• 15 g agar
• H₂O to 1 L.

• Autoclave