Week 1

July 11-

Preparing sent parts

July 12-

Preparing sent parts

growing LB and appropriate antibiotic overnight

July 13-

all grew, negative controls didn't

Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic

July 14-

all grew a blob and a few colonies along the edge

- Transformation at 37 degrees Celsius overnight- 50 µL DH5α, 2 µL DNA (all carb)

Plating sent parts

Take one colony from July 13 plate properly

July 15-

Transformation of 50µL of DH5α, 2 µL DNA (all carb) at 37 degrees overnight

Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm

July 16-

All but negative control grew 12.2.13 is slightly pink probably due to the constant red

Glycerol stock- 350 µL of cells and 150 µL if 50% glyrcerol

Miniprep and transformation results- the plate had about 50 to 60 colonies

July 17-

Incubate in 3 mL LB broth overnight at 37 degrees Celsius and 2020 rpm