Week 3

July 25-

Incubate LB chlora for 1 hour

Negative control continued overnight

Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together. Then plated all 20 µL onto a chlora plate. Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.

July 28-

i2.10.1 – FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate

Incubation FOFR colonies (3 mL of LB and carb)

Plating mutated CheZ (inoculation)

grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB

-grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB

July 29-

Making glycerol stock

Miniprep

Observations: i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow

Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity

July 31-

Incubation of CheZ (I2IT)- 3mL of LB and carb