-Visualization-

@Colony PCR and electrophoresis pSB1C3+oriTr-GFP

@preculture from master plate of Colony PCR pSB1C3+oriTr-GFP

@plasmid extraction and DNA refine OriTr, OriTr-GFP

@plasmid extraction and DNA refine OriTr-GFP-OriTr

@DNA densitometry

OriTr-GFP 264.9 ng/μl

OriTr 126.9 ng/μl

OriTr-GFP-OriTr 139.5 ng/μl

-Electrophoresis

Samples which digested one day ago

-Gel extraction

Above samples

-Ligation

Promoter - suppressed expression system

-Transformation

Above samples

-Targetting-

@Colony PCR J23100-P0140

@electrophoresis J23100-K317037 yesterday emzimed and find binds

@gel extraction, but failure

@electrophoresis J23100-P0140, colony PCR find binds

@preculture J23100-P0140, J23100-K317037