-Targeting-

Plasmid extraction and DNA refine

J23100-P0440 and J22005.

J23100-P0440→７５

J22005→９０

Electrophoresis

Electrophoresis J23100-K317037 and J04450 that yesterday emzimed.J23100-K317037 find binds.But J04450 failure.

Gel extraction

J23100-K317037,J22005.

J23100-K317037→１９０

J22005→１６３

Ligation and transformation

J23100-K317037 and K283030,J22005 and J22005.

Restriction enzyme

J04450,J23100-P0440.(EcoRⅠ, Pst)