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Digest of pUCBB-lacZ, pBBR, and ompR

Project: Regulatory

Date: Sat, 10 Sep 2011 13:06:54 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

• Sat, 10 Sep 2011 13:11:38 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Purpose: to digest pUCBB-lacZ, pBBR, and ompR with suitable restriction enzymes to facilitate replacement of pBBR promoter with ompR promoter. Because pBBR is a low copy number plasmid, we have elected to perform the same steps in tandem with pUCBB-lacZ which is a high copy number plasmid.

Procedure:

The following digestion reactions were set up:

• 16 ul ddH2O

• 3 ul NE buffer 2

• 1 ul XbaI

• 1 ul BglII

• 10 ul ompR

• 16 ul ddH2O

• 3 ul NE buffer 2

• 1 ul XbaI

• 1 ul BglII

• 10 ul pUCBB-lacZ

• 16 ul ddH2O

• 3 ul NE buffer 2

• 1 ul XbaI

• 1 ul BglII

• 10 ul pBBR

Results: None

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