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Data

TetR Mutants

• V36F
• P39K
• Y42F
• P39Q-Y42M

T7 Promoter Plasmids

• pSB3K1-T7-const-RFP
• pSB3K1-T7-lac-RFP
• pSB3K1-T7-lac2-RFP
• pSB3K1-T7-const-Lysis
• pSB3K1-T7-lac-Lysis

• pSB3C5-T7-const-RFP
• pSB3C5-T7-lac-RFP
• pSB3C5-T7-lac2-RFP
• pSB3C5-T7-lac-Lysis
• pSB3C5-T7-lac2-Lysis

T7 Promoter Variants

Reporter Plasmids

J61002 Ptet-RFP

TetR plasmids

pSB3K1 Pconst-TetR

Parts assembled:

• backbone: pSB3K1 from the registry http://partsregistry.org/wiki/index.php?title=Part:pSB3K1
• Pconst, including RBS and spacer