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Team:UQ-Australia/Project
From 2011.igem.org
| Inspired by the circadian clock in humans which regulates a number of very important processes, we are trying to replicate this biological clock in a bacterial system. We are aiming to construct a network of genes that oscillates in a similar fashion to the 24 hour system in humans. If we are successful, we will be able to put different genes into our system so that we can make the bacteria perform a particular process periodically – a simple example of this would be to make them flash on and off consistently. | |
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To achieve this oscillatory behaviour we will utilise a gene network with a series of inducible promoters that generate the production of other activating proteins, all driven by a constitutively active promoter. This promoter features an engineered repression domain (the inhibitor of this promoter being the output of the final step in the network). If everything goes as planned, these linked activations and repression will produce fluctuating levels of the proteins in question, which could then be used to drive our output function (initially just GFP production and a timed fluorescence). Ultimately, we hope our system could be used to drive the timed release of drugs or other biological factors. | File:UQ-Australia team.png Your team picture |
| Team Example |
Project Details
The project has been split into categories:
- Development of BioBricks
- Experimental methods to be fully recorded in the Notebook
- Modelling of the circuit
- Modelling of the kinetics of the oscillating cells
- Modelling of the synchronisation of oscillating cells
- Thorough evaluation of the safety issues regarding UQ-Autralia's entry in iGEM
- Human practices
- Raising awareness of synthetic biology
- Providing a solution to the patenting issue that iGEM is facing
Together, this forms the UQ-Australia project for the 2011 iGEM.
Experimental Work
Outcomes of experimental work are to be recorded here.
Modelling
Details of this is on the Modelling page.
Safety
Details of this is on the Safety page.
Human Practices
The Human Practices section is on the Human Practices
