Team:Paris Liliane Bettencourt/Notebook/2011/08/29/

From 2011.igem.org

Revision as of 12:40, 29 August 2011 by Cyrpaut (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Team IGEM Paris 2011

Cyrille

Digestion

New attempt of ligation and destroying the next PstI site.

2 times 500ng of S24 was digested in SP for 3h 2 times 500ng of QCP YFP TetR was digested in P for 3h 500ng from the tubes 1 and 6 was digested in XP for 1h and the digestion runned on a gel.

A gel extraction of the band was carried on the lione 1. The result is about 9ng/µL.

ladder - 1 - 6
Cutted band

Ligation

Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/08/29/"