Team:UNICAMP-EMSE Brazil/protocols/Digestion

Digestion:

(For each 1000 ng of DNA use 1 U of enzyme in 20uL reaction)

1. Use 3000 ng BioBrick vector
2. 6 µL 10x buffer
3. 0.5 µL enzyme 1 (20 units/µL)
4. 0.5 µL enzyme 2 (20 units/µL)
5. distilled water to 60 µL total volume

• Put in 0,2 mL eppendorf tubes and let 2 h at 37°C.
• Make an electrophoresis (use the hole reaction volume) and cut the band with your gene of interest.