Team:Arizona State/Lab/Protocols/Media

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Protocols: Media


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SOB / SOC


"Super optimal broth" (SOB) and "super optimal broth with catabolite repression" (SOC) are nutrient-rich media. Use extreme caution (and if possible make only small amounts) when manipulating, as they are very easy to contaminate.

For 1 L SOB, combine:

  • 20 g Tryptone
  • 5 g Yeast extract
  • 0.5 g NaCl (8.56 mM)
  • 1.86 g KCl (2.5 mM)
  • ddH2O to 1 L

Autoclave.

SOC: Add to SOB:

  • 0.952 g MgCl2 (10 mM) or 2.408 g MgSO4 (20 mM)
  • 3.603 g glucose (20 mM)

Do not autoclave SOC, as the glucose will react with the tryptone. Either autoclave glucose separately or filter sterilize the SOC.

LB agar


  • Combine 3.7 g LB agar with 100 ml H2O. Autoclave or microwave. Makes ~4 plates.

LB broth


  • For 1 L, add to 800ml water:
  • 10 g tryptone
  • 5 g yeast extreact
  • 10 g NaCl
  • Adjust ph to 7.5
  • Adjust volume to 1 L
  • Autoclave

Trypticase soy broth


Trypticase soy agar


  • For 1 L:
  • 30 g soy broth
  • 15 g agar
  • H2O to 1 L.
  • Autoclave