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From 2011.igem.org

Safety

Safety to both scientists and the general public is of utmost importance to anyone working in the synthetic biology field. We stringently followed all relevant safety protocol as well as choosing the safest procedures and materials to work with.

Material Safety

Researcher Safety
We were able to design a project that did not involved the use of many toxic chemicals. The chemicals we used that presented a potential threat to researcher safety were: ethidium bromide, for visualization during gel purification, and SU-8 photoresist and developer, in order to construct the mask used to make our microfluidic device. To protect researchers when using ethidium bromide, all researchers were required to wear gloves when handling EtBr. After handling toxic chemicals, researchers were required to discard contaminated items, such as gloves, so as not to spread contamination. Gels containing EtBr were properly discarded in a biohazard bin. Exposure to UV light during viewing of gels was minimized through the use of safety masks covering the eyes and face.

Public Safety
Our projects presented little to no risk to public health or safety. Our E. coli strains are non-pathogenic. Our bio-chemical pathway converts tryptophan to prodeoxyviolacein. Both chemicals and all intermediates are non-toxic. We chose this pathway because tryptophan is both common and harmless to public safety.

Environmental Safety
Our E. coli lab strain is not able to survive outside the lab and were disposed of safely. Our project also poses no identifiable threat to environmental safety. All bio-hazardous chemicals were disposed of by following the proper regulations.

Security Risk
There are no real threats that can be created from our project since our final product is non-harmful. A person with malicious intent could possibly create a biochemical pathway that creates a harmful product but such technology is already widely known and out of our control.

Safety of BioBricks
None of the BioBricks submitted to the registry raised any safety issues. They are GFP, RFP VIOA, VIOB and VIOE attached to avitags. Our last BioBrick part causes cells to lyse in the precence of green light. This should not cause any safety concerns.

Biosafety Provisions

Biosafety Rules and Procedures
We complied with Weill hall’s safety requirements in gaining access to lab space, as well as in use of the lab. All safety information and procedures are linked on the main Weill safety page [http://blogs.cornell.edu/whfs/weill-hall-safety-links-and-information/ here].

Weill Hall Safety Personnel
Scott D. Emr is the director of Weill Hall which is the building our lab is located. There is also a Weill Hall Safety Committee. While we did not personally meet as a group with either groups, we talked Dr. Archer who is in charge of our particular lab space. She approved of our project and helped us with the safe construction of our microfluidic mask.

Safety Training We received safety training from two online courses that all members were required to pass in order to gain access to the building. These were Lab Safety and Chemical Waste Disposal. Also we received training from our lab instructor on basic safety issues such as waste disposal, use of the fuse hood, etc.