green light receptor
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blue light receptor
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red light receptor
3A-assembly
Investigators: Sandra
Digstion
- PR1 (pSB1C3)
- PR2 (pSB1C3)
- pcyA (pSB1T3)
- pcyATd (pSB1T3)
- pcyATb (pSB1T3)
- L23 (pSB1T3)
- vector (pSB1K3)
Amount of DNA and H20:
Sample
| 500(ng)/DNA concentration (ng/μl)
| H20 μl
|
pcyATd
| 2.8
| 35.2
|
pcyaTb
| 2.9
| 35.1
|
PR2
| 7.2
| 30.8
|
PR1
| 6.1
| 31.9
|
L23
| 4.8
| 33.2
|
pSB1K3
| 20
| 18
|
Enzymes necessary for digestion:
| PR1/PR2
| pcyA/L23
| vector
|
enzyme 1
| EcoRI
| XbaI
| EcoRI
|
enzyme 2
| SpeI
| PstI
| PstI
|
-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C
Ligation
Lysis cassette
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Precipitator
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