• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

Microfluidics-work in progress

For the in vivo and in vitro characterization of our mutant transcription factors, we used two high-troughput microfluidic devices, a cell culture chip and respectively a fluidic platform for measuring molecular binding events, called "MITOMI".

The field of microfluidics has as an objective the manipulation and analysis of fluids within micrometer-sized channels, which, due to the small size, must be microfabricated using techniques adapted from the semiconductor and plastics industries.

The advantages of this technique include:

• low sample and reagent volumes leading to lower costs low waste levels
• unique physical properties of microscale fluid flow
• the reduction of the size of equipment
• fast analysis and short reaction times
• parallel operation for multiple analyses
• possibility of portable devices.