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From 2011.igem.org

Tuesday

Labwork

• Run gel on the PCR products from yesterday - to see if the new primers work better so we now will se an insert. The gel showed a positive result for A1 and A2 in the expr. vector.

• Make MiniPrep on the O/N cultures from yesterday which appear to have an insert - we have picked two of each of A1 and A2 which showed a posítive gel result.

• Do digestion of PSB1C3 once again, since the digestion O/N was to be in 37 degrees.