"
Team:Sevilla/Week2
From 2011.igem.org
- Home
- The team
- The project
- Software
- Modelling
- Safety
- Notebook
- Sponsors
- Acknowledgments
- ArcanumTV
- Gallery
Monday 18 July
Our inoculums look pleasantly cloudy, as expected. All except two. We freeze samples of the good ones in glycerol at -80ºC in a wonderful fridge that could house a corpse(who knows?).
We start studying the protocols of our miniprep kit to purify the BioBrick plasmids from our transformed cultures. They don't seem to be extremely complicated but we have to do about 50; shoulders to the wheel!
We receive several BioBricks and strains we had ordered; vainillin and rhlR BioBricks, two strains of B.subtilis, another strain containing VanM/N genes... people are being quite kind to us! We seed the bacteria and transform with the BioBricks, depending on the format thay come in.
Our minipreps are ready at last after a hard work day, and we finally leave some inoculums of DH5-a to prepare competent cells tomorrow. 21.30. Lab OFF.
Monday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Tuesday 19 July
Materials and chemicals keep coming in big cardboard boxes full of foam rubber (we're storing all that foam to fill a bath next month and have some fun).
More good news: our transformed cultures have grown as expected, as well as the B. subtilis strains. At the end of the day we make inoculums of them all so that they grow o/n and tomorrow we can freeze samples and do MORE minipreps (we have just started and we're already sick and tired of that kit...)
We need to discuss which assembling method we'll use with the BioBricks. It takes us some hours, and in the mean time Andrés faces the personal challenge of making competent cells from the DH5-a inoculum with the new protocol. Tomorrow we'll see who wins the battle...
And Pedro isn't wasting time at all, he's just found out racks are stackable as Lego pieces. This discovery has changed his life, look how happy he is!
After deciding how we'll build each BioBrick and counting all the ligations we need to do, we finally tackle the digestions with the different restriction enzymes. Mastermix kits. More protocols. GREAT.
Tuesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Wednesday 20 July
AT LAST our official mascot is here! Say hello to Mr. Gibss!Rocío had it kidnapped in her house, but to make up for that, she's bought a WONDERFUL Disney princess clock to cheer our lab up!. Giving the lab a home-loving touch.We have also brought a coffee machine, we need caffeine in industrial amounts to work here for so many hours a day.
More minipreps and frozen samples. But we're also trying something new! Our first electrophoresis, to see how the digestions and the minipreps go. We're examining the results. It's ok to be the first time, but there's so much to improve yet...
Marta is in charge of our first ligations, tomorrow we'll see how they went.
...and how did the great battle end? Andrés 1 - E.coli 0. We have competent cells!
Out of the lab before 19.00. What a record!
Wednesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Thursday 21 July
We run an electrophoresis of the digestions we made yesterday, but it hasn't worked propperly, it seems the process needs fine tunning.
We spend some time preparing the rewards for those who gave us money for the project through the crowdfuning platform. Letters, funny badges, videos...
Coffee break while we run one more electrophoresis...
We have a nice visit today! Lauren, from the team of Pensylvania, is here telling us about her project and learning a bit more about ours. Carlos is having an intense discussion on kinetics with her while Marta struggles to design the primers we'll need for the PCR reactions next week. There's no way to get them right, it's such a nightmare!
Thursday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Friday 22 July
It seems nothing's gone right so far except for the transformations. We'll have to start over...BUT WE HAVEN'T SAID OUR LAST WORD YET! [To be continued...]
Friday Protocols
Weekend
Blondie and little Rosidito didn't appear in the lab these two days so our bacterial cultures died and we have to lose time on Monday making new bacterial cultures. The rest of the group are starting to hate both clumsy girls. Tristique, mauris turpis? Arcu tristique sed massa est nunc purus pellentesque, risus nec rhoncus, scelerisque ac urna porttitor, sit.
Weekend Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
