Team:Harvard/Technology

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Chip Synthesis

We are creating 55,000 zinc fingers using microchip synthesis (Kosuri et al). These fingers will then be tried against the DNA sequences we wish to bind.

Getting zinc finger DNA off of a chip (Kosuri et al)

MAGE

References

Sriram Kosuri, Nikolai Eroshenko, Emily M LeProust, Michael Super, Jeffrey Way1, Jin Billy Li, George M Church. Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nature Biotechnology: http://www.nature.com.ezp-prod1.hul.harvard.edu/nbt/journal/v28/n12/full/nbt.1716.html .