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Suspension cell cultures

As we are dealing with bacteria, work under a flame!

First, prepare the medium in a falcon tube. One tube per colonie you will pick:

• 5 ml LB medium (be careful not to touch the walls of the bottle!)
• 5ul ampicillin (or other antibiotic)

Then, for each colonie, drop a tip in it (don't make a hole in the agar) and put the tip in the tube. The tubes should be incubated overnight at 37°C; put the tubes 30°C inclined.