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Glycerol stock

Material needed: overnight cultures of transformed cells (do not centrifuge them) and glycerol 50%

• Mix gently the cell cultures to resuspend the pellet
• In an Effendorf tube, put 500 ul of glycerol 50% and 500 ul of cell culture (always keep 1:1 ratio)
• Label the tube
• Mix gently and put the Eppendrofs quickly in the -80°C freezer (glycerol is toxic for the cells)