Team:Northwestern/Notebook/Protocols/Overnight

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RETURN TO IGEM 2010

Overnight

REMEMBER TO USE PROPER STERILE TECHNIQUE


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube 
  *MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER

[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware

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