• Take 5mL LB and put in a round bottom 5mL tube.
• If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube. Make sure you add the right one!
• Take a p200 tip and lightly touch a colony.
• Eject the tip into the culture and put the cap on.
• For a miniprep, grow overnight for 12-16 hours.
• Left over overnight cultures (after miniprepping) can be used to make glycerol stocks.

REMEMBER TO USE PROPER STERILE TECHNIQUE

Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]