Team:UTP-Panama/After Regional Week 1

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October 17 to 23

October 18th

INVENTORY:

Quantity Description
24 petri plates (60 x 15 mm)
a lot! eppendorf tubes of 0,5 mL
4 tubes with linearized plasmids (kit)
8 tubes with competent cells
- genetic material from minipreparations
? ligation kit
2 tubes with SOC media (15 mL)
2 tubes with cloramphenicol (liquid or powder)
2 petri dishes with GaTech colonies
1 petri plates with RFP colonies
1 jar MgCl2 (for doing comp. cells)
1 jar CaCl2 (for doing comp. cells)
1 jar with ultrapure water
3 jars minipreparation Buffers (P1,P2,P3)
3 boxes small pipette tips (10 uL)
4 boxes medium pipette tips (200 uL)
3 boxes large pipette tips (1000 uL)
35 falcon tubes of 15 mL

Missing:
- large gloves
- falcon tubes 50 mL
- antibiotics
- LB media
- Eppendorf 2 mL for auto-nailing

October 19th

Ricardo reviewed the inventory and provided us:
- 1 box of large gloves
- 1 bag with falcons 50 mL
- 1 bag with eppendorf of 2 mL

Also:
- solid LB works
- not necessary to do liquid LB (there is)
- we have to transform competent cells with:
- Bristol (the plate isn't so good)
- RENBO (the product was ligated in the ampicillin plasmid) --> to do minipreparation and electrophoresis
- Colonies only without plasmids.

Plates with RFP and GaTech are fine!

We had to:
- prepare new antibiotics
- auto-nail the eppendorf of 2 mL, they were ready.


- prepare the Minimal Media for the experiments.
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