Team:UTP-Panama/Week 16
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | After Regional Week 1 | After Regional Week 2 | Week 16: September 19 to 24September 19WET LABI. DIGESTIONFor each Eppendorf PCR was added the following: There was added the 6 components to the small tube of PCR. 7. Two (2)eppendorf tubes were incubated in the thermocycler during 1 hour at 37°C : Transformation of Competent CellsA.
4 petri dishes were filled with 5mL of solid LB
3 positive controls and 1 negative control.
Total: 19.36µL para 20µL de LB 'Content of controls:' 1. In 3 eppendorf tubes was added the specified amount in positive control and were resuspended. September 20Scientific Comunication ActivitiesTree sessions: WET LABObjetives or Title: 1 2 3 4 1, 2 : BBa_K381001 (positive control) 3, 4 : BBa_K381001 (negative control) September 21WET LABThis day the electrophoresis test was performed to the obtained plasmids with BBa-K381001 in Sept 14, 2011. September 23WET LABWe carried out a dirty mini prep protocol to BBa-K672000 to send 250ng DNA in 10µL TE. A sample was preserved for electrophoresis. September 24GENERAL SESSIONAfternoon: Talikng about WET LAB final activities.
Design of the "SB UTP 1.0" and "SB UTP Project 1.0" |