Agarose gel electrophoresis

Written on 01.07.2011

Purpose

To analyse DNA composition of a sample. Voltage-dependent DNA migration through agarose gel allows sequences separation according to their size.

For a 1% agarose gel

For sample loading: RedSafe

To prepare the gel:
- Weight agarose, place it into a bottle with a cap, add TBE 1x buffer

• Place the bottle into a microwave, <gras>loosen the cap</gras>, heat it untill it almost boils and the agar is dissolved

• Cool it down until it can be hold (that can be done under water)

• Add 1.25µl of GelRed

• Pour into the rack, put the comb and wait until it polymerizes