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Agarose gel electrophoresis

made on 01.07.2011

Purpose

To analyse DNA composition of a sample. Voltage-dependent DNA migration through agarose gel allows sequences separation according to their size.

Ingredients

for a 1% agarose gel

Method

• Weight agarose, place it into a bottle with a cap, add TBE 1x buffer

• Place the bottle into a microwave, <gras>loosen the cap</gras>, heat it untill it almost boils and the agar is dissolved

• Cool it down until it can be hold (that can be done under water)

• Add 1.25µl of Gel Red

• Pour into the rack, put the comb and wait while it polymerizes

Usually “B” valves are starting to load earlier (leave the button unpressurised prior to the pressure increase, it tends to stick to the slide.)

Increase the pressure in both “A” and “B” valves till ~13-15 psi and make sure that you can see that all chambers are separated. If no – increase the pressure