Team:Harvard/Results/Acknowledgements
From 2011.igem.org
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Team Acknowledgements
Our project was selected from a pool of 10 possible project ideas submitted by both team members and advisers. Team members decided to focus on zinc finger proteins because of the technologies (chip DNA synthesis and MAGE) available to us: we believed that developing new zinc finger proteins was a way to use both technologies to their greatest possible extent. The original idea of zinc finger proteins was contributed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li.
This project was made possible by support from the George Church lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology. Without their valuable guidance and great patience, we never would have been able to achieve what have done so far.
Although the project is the first to utilize several key technologies in novel ways, the following technologies were developed outside of iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al in the Church lab. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al in the Church lab, and the actual oligo synthesis was generously provided by Agilent Technologies.
We owe great thanks to Alain Viel for his flawless management of the logistics of iGEM, including but not limited to providing lab space, ordering needed lab supplies, arranging our trip to the Americas Regional, and for his excellent taste in footwear. Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.
Team Member Acknowledgements
All project work was done by team members, and we would like to make the following acknowledgements:
Kristin Barclay
Bioinformatics, chip sequences design and programming, majority of wiki coding and design
Justin Chew
Bioinformatics, ZF target selection, ZF expression plasmid construction, Wolfe selection system testing, wiki coding and design, poster design
William Clerx
Bioinformatics, ZF target selection, wiki coding and design, video editing, freelance wet lab
Sarah Choudhury
TolC selection system construction, TolC and Wolfe selection system testing, biobrick construction
Naomi Genuth
Wolfe selection system construction and testing, biobrick construction and registration
Brandon Gerberich
Bioinformatics, Wolfe selection system construction and testing, interview cameraman and video editor
Mark Kopelman
Wet lab
Matt Lunati
TolC selection system construction and testing
Nida Naushad
Bioinformatics, chip sequences design and programming, ZF expression plasmid construction, chip library assembly, biobrick construction
Adviser Acknowledgements
Finally, we would like to specially acknowledge all of our teaching fellows (TFs) who have always been there for us, no matter how small or big the problem. Their advice has been of immeasurable value to our project's success. We would also like to also acknowledge the following TFs for their special contributions:
Daniel Goodman
Harvard iGEM logo design, wiki design (contributer to CSS and design of the navigation bar), and being an all around awesome team leader
Noah Taylor
Used lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical, contributed a reverse translating function from his own research to bioinformatics
Srivatsan Raman
Collaborator in our project abstract and Human Practices page