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Team:WITS-CSIR SA/Project/Achievements
From 2011.igem.org
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1) We have submitted 11 biobrick parts to the Registry of Standard Biological Parts. Three new biobrick parts representing our “favourite” biobricks, BBa_K537001, BBa_K537002 and BBa_K537003, have been fully characterized.
2) We are the first team to characterize the function of two different types of theophylline-responsive riboswitches, and this was achieved for two separate coding sequences. These riboswitch designs are improvements over previously-developed iGEM riboswitches.
3) Theophylline-response riboswitch 2 (thRS2-CheZ, BBa_K537002) was capable of significantly higher activation than Theophylline-response riboswitch 1 (thRS1, BBa_K537001) when expressing motility factor CheZ (37-fold vs 4-fold). Moreover, thRS2 showed 6-fold improved motility function over thRS1 and 1-fold increased chemotactic sensitivity to theophylline.
4) Theophylline-responsive riboswitch 2-CheZ (BBa_K537002) represents an ideal biobrick for N-terminal fusion to proteins of interest for theophylline-activated messenger activity. This part could have numerous advantages in several other synthetic biology applications.
