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<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Protocol

Protocol

Biofilm Formation

Biofilm Formation Protocol with common lab equipment:

AxyPrep™ Plasmid Miniprep Kit

AxyPrep™ PCR Cleanup Kit

TaKaRa Agarose Gel DNA Purification Kit Ver.2.0

Chemically competent E.coli preparation.

Transformation

Colony PCR:

Agarose gel electrophoresis