-Targeting-

PCR

PCR for J2005

-Electrophoresis

Checked for samples of PCR

Could not check

-PCR (2nd)

PCR for J2005

-Electrophoresis (2nd)

Checked for samples of PCR

Could not check

-Preculture

Precultured for K283030 and K317037, incubated at 37 deg C

-Transformation

Transformed J23100

then incubated at 37 deg C