-Visualization-

@plasmid extraction oriTr-GFP-oriTr and JM109

@plasmid extraction and DNA refine oriTr-GFP-oriTr and JM109

@DNA densitometry

oriTr-GFP-oriTr 5 26.1 ng/μl

oriTr-GFP-oriTr 6 51.3 ng/μl

oriTr-GFP-oriTr 7 47.2 ng/μl

oriTr-GFP-oriTr 8 58.4 ng/μl

@restriction enzyme to oriTr-GFP-oriTr (XbaⅠ, PstⅠ)

@electrophoresis and gel extraction oriTr-GFP-oriTr5～8 and pSB1C3(restriction enzymed 9/15)

@DNA densitometry

totaled oriTr-GFP-oriTr5,6,8 18.4 ng/μl

pSB1C3① 16.5 ng/μl

pSB1C3② 11.5 ng/μl

@electrophoresis for confirmation, but could not find bind.