-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmids and refined DNA for P0140, J23100-K317037-K283030 (John-F-Kennedy) and pSB1C3 used Cell Biochemical Lab's kits.

Results (concentration = ng/ug)

P0140

I. 198

II. 105

John-K-Kennedy

I. 135

II. 170

pSB1C3

I. 140

II. 173

-Treat restrction enzyme

Treated restriction enzyme for refining DNA and GFP.

Treated with X and P. After, incubated at 37 deg C.