Team:Tokyo Metropolitan/Project

Targeting Device gives new chemotaxis to E.coli. With this device, BeE.coli go for the target bacteria concentrated area. It is constructed to make chassis move in straight line when it receives a kind of chemicals. If the target bacteria produces a specific chemical substance, you can make chassis to swim for target bacteria by putting a appropriate promoter into this device.

Project "BeE.coli" We propose the project "BeE.coli" as the micro injection system. We will make E.coli that moves in high speed to the target and kill it. We have been trying to put three different facility such as "speed up", "targeting", and "killing" by enhancing the flagellar movement, taking control of flagellar rotation, and sending killer gene to the target.
	Speeding Up Device makes E.coli to move in higher speed than usual. E.coli have HNS protein gene in their genome. HNS is an Escherichia coli nucleoid protein known only to function as a modulator of gene expression. Gina M. Donato said that specific single amino acid substitutions in H-NS caused an approximately 50% increase in flagellum rotational speed. So we have E.coli to express HNS mutant T108I gene with plasmid.