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Copenhagen/28 June 2011
From 2011.igem.org
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Thuesday
Status
It seems that something went worng with our mutants from last week since we weren't able to transform XL1-Blue with them - even though we used a larger amount of plasmid. So now we put all our faith in the mutants we made yesterday.
Lab work
- Finished the mutation: Digested the parental plasmid. Protocol Mutations
- Transformed E.coli with our new mutant plasmids. Protocol Transformations
- Took one colony out of our XL1-Blue transformed with biobrick no. BBa_J04500 [http://partsregistry.org/Part:BBa_J04500] and placed it in an overnight culture.
Tomorrow
We will do Real Fast Mini Preps of our BioBrick and hopefully be able to pick a colony transformed with our cyp for an overnight culture.
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