Team:Osaka/week8
From 2011.igem.org
Contents |
September 18(Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- To liquid culture:03,105,106,107,108,109,110,111,112
September 19(Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 113(T):105(upstream)+110(downstream)+1-7A(vector)
- 114(T):111(upstream)+112(downstream)+1-7A(vector)
- Transformation of ligation products
- Plating (cell survival)
September 20(Tue)
- To liquid culture:113,114
- colony check (cell survival)
September 21(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Ligation
- 115(A):113(upstream)+114(downstream)+1-1G(vector)
- Transformation of ligation products
- colony check (cell survival)
- Plating (cell survival)
September 22(Thu)
- Colony check
- Plating
- To liquid culture
September 23(Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- ok
- Colony check
September 24(Sat)
- Preparation of LB plates
- Plating