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Protocols
Home
iGEM 2011 Home
Project
Introduction
CRISPR
E. coli
B. halodurans
L. innocua
Software
Future
References
Lab
Team
Photos
Protocols
Safety
Acknowledgements
Results
Data
BioBricks
Notebook
April/May
June
July
August
September
Cas PCR Log
Sequence Information
Other Documents
Human Practices
Events
Collaborations
Exploring Synthetic Biology
Outreach in Practice
Competency
:
NEB cells
TSS cells
Assembly Methods
:
SLIC
Poly-X ligation
Ginkgo assembly
Biobrick assembly
DNA Extraction
:
Ethanol precipitation
Gel extraction
Miniprep
Genome prep
Gel visualization
Media
:
SOB / SOC
LB agar
LB broth
Trypticase soy
PCR
Restriction
Ligation
Transformation
:
NEB cells
TSS cells
Culturing cells
:
Liquid culture
Plating cells
Glycerol stock
Buffer solutions
: