Team:Calgary/Notebook/Protocols
From 2011.igem.org
Protocols
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Plasmid Extraction (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Conjugation of E. coli plasmids to Pseudomonas spp..
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-LacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the LacI-LacZ I732901 gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing chemically competent cells (E. coli)
- Preparing glycerol stocks (E. coli)