Mutagenesis PCR

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Mutagenesis PCR was done using the protocol established by agilent's [http://www.genomics.agilent.com/files/Manual/210514.pdf Quiklightning Multi-site directed mutagenesis kit.]

Our Mutagenesis Primers were:

1. 5' - CGAATCACGTGCTACAGCGCGCCTACG - 3'

2. 5' - ACCTGCCGCACTGTAGTTTCTCGCGGG - 3'

3. 5' - CTCGACCGAACACCTACAGGGCGACTTCTAC - 3'

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