Our project uses 3 major new technologies: Multiplex Automated Genome Engineering (MAGE), chip-based synthesis of DNA, and lamba red recombination, along with more traditional bioinformatics. We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.

Bioinformatics

See our Project:Bioinformatics page for details on the computational aspects of our project technology.

Zinc Finger Binding Site Finder

Check out our Zinc Finger Binding Site Finder Tool here! This tool was designed and used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.

Chip-Based Synthesis

We are creating 55,000 zinc fingers using microchip synthesis. These fingers will then be tried against the DNA sequences we wish to bind.

• Original Paper: http://www.nature.com/nbt/journal/v28/n12/full/nbt.1716.html
• Our protocols for getting DNA pools off of the chip: https://2011.igem.org/Team:Harvard/Protocols#Chip_DNA_Extraction

MAGE

Multiplex automated genome engineering (MAGE) is a new method for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome, thus producing combinatorial genomic diversity.

• Original Paper: http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html
• Our Protocols: https://2011.igem.org/Team:Harvard/Protocols#MAGE

Lambda Red- Mediated Recombineering

Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.

• Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
• Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement
• Our Protocol: https://2011.igem.org/Team:Harvard/Protocols#Lambda_Red

Gibson (Isothermal) Assembly

An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase.

• Original Paper: http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html
• Protocol: http://www.nature.com/protocolexchange/protocols/554#/main
• Our Protocol: https://2011.igem.org/Team:Harvard/Protocols#Isothermal_assembly