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Glycerol Stock
From 2011.igem.org
Materials:
Cell culture
40% glycerol solution
Procedure:
1. Grow an "overnight" cell culture of the desired sample.
2. Add 1ml of "overnight" culture into a 2ml microtube or cryovial.
3. Add 1ml of 40% glycerol solution into the same tube.
4. Store at -80°C.( To reanimate simple inoculate 50ul into fresh LB broth with the appropriate antibiotic)
