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Violacein
We plan to finish ligating the 5 violacein expression cassettes into the PRS416 Yeast Vector and transform it into a URA3 Knockout yeast strain.
We plan to carry out the random ligation to make the promoter/violacein ORF/3'UTR/loxPsym combinatorial library and transform into synIXR yeast to get varying levels of violacein expression.
Characterizing the Promoters
We have successfully transformed a number of promoters (BAP2p, TDH3P, KRE9p, HHO1p, PRY1p). We have also successfully transformed reference promoter constructs into yeast. We plan to proceed to flow cytometry analysis in the near future. We also plan to transform our other promoters into yeast. We hope to conclude this part of the project before the Americas Regional.
