Team:UNICAMP-EMSE Brazil/Methodology

Creating a great WARRIOR!

In order to create our “Jedi coli” to fight the STRESS WARS, we trained padawan colis to use their power to sense Nitric Oxide (NO) or Adrenaline and produce IL-10 and IL-12, respectively, to defend or bodyConfederation host against inVader bacteria. By the end of the harsh Jedi training, which included hours of cutting and ligation, cloning and transformation, we aimed to develop the following gene constructions:

Work plan:
Our work was divided in three main steps:

1. Biobrick assembly;
2. Suit the biobrick for shipment (remove them from the original plasmid and insert them on pSB1C3 plasmid);
3. Devices construction and testing.

First step: Biobricks Assembly