Team:Harvard/Results/Chip Synthesis

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Overview | Biobricks | Source Code | Acknowledgments | Accomplishments

qPCR curves

When performing a qPCR, one wants to run the reaction while the growth is still exponential. During this phase, all the oligos are replicated at equal rates. Once growth levels off, the reaction should be stopped as the oligos are now being replicated unequally. Each of the graphs below represents a qPCR for one of our sub-pools.

HARVQpcr.png

Sequencing results of Library transformation

Error rates

Still being updated

  • Good: 57.8% (22/38)
  • Single SNP: 5.3% (2/38)
  • Multiple SNPs: 18.4% (7/38)
  • Frame shift: 18.4% (7/38)

Distributions

Still being updated Each of the sequenced zinc fingers have original F1 fingers.

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