Team:UC Davis/Data LacI

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LacI Promoter Characterization Data

Wild-type

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99353, 0.78661, 0.61233, 0.48347, 0.40302, 0.31833, 0.17311 0.0031579, 0.0052541, 0.0059555, 0.0069092, 0.0041919, 0.0052347, 0.0024241 4 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.99353, 1.02920, 1.05770, 1.12136, 0.78661, 1.00142, 1.02352, 1.06377, 0.61233, 0.90829, 0.95385, 1.06676, 0.48347, 0.77363, 0.85365, 0.99591, 0.40302, 0.67853, 0.74660, 0.93119, 0.31833, 0.56058, 0.62386, 0.83851, 0.17311, 0.33178, 0.40330, 0.58631 0.0031579, 0.0078785, 0.0087252, 0.0491108, 0.0052541, 0.0065114, 0.0028587, 0.0289843, 0.0059555, 0.0047101, 0.0029583, 0.0241440, 0.0069092, 0.0036622, 0.0052977, 0.0270465, 0.0041919, 0.0042828, 0.0210219, 0.0054843, 0.0052347, 0.0067734, 0.0282990, 0.0027105, 0.0024241, 0.0038951, 0.0043371, 0.0029370

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.84293, 0.52501, 0.28185, 0.14559, 0.082367, 0.05077, 0.017707 .017132, .016563, .010138, .0083723, .0036696, .0013295, .00029933 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.84293, 0.87306, 0.88569, 0.88705, 0.52501, 0.73231, 0.85955, 0.86384, 0.28185, 0.49594, 0.754, 0.85027, 0.14559, 0.30866, 0.53435, 0.79143, 0.082367, 0.20109, 0.38603, 0.6632, 0.05077, 0.13482, 0.28296, 0.59708, 0.017707, 0.062278, 0.080463, 0.22066 0.017132, 0.017727, 0.020661, 0.014524, 0.016563, 0.0095391, 0.013332, 0.024361, 0.010138, 0.017069, 0.017257, 0.019877, 0.0083723, 0.0064858, 0.006745, 0.035387, 0.0036696, 0.0024604, 0.014455, 0.017886, 0.0013295, 0.0017976, 0.0039159, 0.011506, 0.00029933, 0.0014914, 0.00096534, 0.0054255

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.66043, 0.44899, 0.27847, 0.19007, 0.14271, 0.11669, 0.061421 .018716, .015384, .0098251, .011268, .0061044, .0030215, .0008294 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.66043, 0.72139, 0.72156, 0.68292, 0.44899, 0.6366, 0.71926, 0.69494, 0.27847, 0.45878, 0.63883, 0.68131, 0.19007, 0.33367, 0.48602, 0.61718, 0.14271, 0.2557, 0.3774, 0.53217, 0.11669, 0.19954, 0.29437, 0.4718, 0.061421, 0.096767, 0.1356, 0.25018 0.018716, 0.012137, 0.012946, 0.017598, 0.015384, 0.012073, 0.011769, 0.010884, 0.0098251, 0.014087, 0.005962, 0.016007, 0.011268, 0.010077, 0.0063601, 0.032621, 0.0061044, 0.010882, 0.0061672, 0.029959, 0.0030215, 0.0073785, 0.00397, 0.01491, 0.0008294, 0.0070557, 0.0015888, 0.0026091

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.63328, 0.35345, 0.1976, 0.1104, 0.065516, 0.042821, 0.014963 .033565, .014178, .0098194, .011065, .0057402, .0026293, .00076312 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.63328, 0.69271, 0.71026, 0.75696, 0.35345, 0.51328, 0.67568, 0.7587, 0.1976, 0.3267, 0.50067, 0.70773, 0.1104, 0.20677, 0.33992, 0.52743, 0.065516, 0.13261, 0.23971, 0.41148, 0.042821, 0.085832, 0.16709, 0.35498, 0.014963, 0.022957, 0.035527, 0.12963 0.033565, 0.036371, 0.039807, 0.021793, 0.014178, 0.0060065, 0.023267, 0.019054, 0.0098194, 0.0058528, 0.0066284, 0.015857, 0.011065, 0.0034758, 0.0039971, 0.064364, 0.0057402, 0.0017609, 0.0055395, 0.040444, 0.0026293, 0.0013106, 0.0046305, 0.012042, 0.00076312, 0.00032783, 0.00066923, 0.0016036

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.31767, 0.17005, 0.10219, 0.063898, 0.045055, 0.034767, 0.017343 .010988, .0044378, .0031212, .004087, .0025152, .00043641, .00020276 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.31767, 0.34757, 0.3608, 0.34776, 0.17005, 0.25419, 0.33186, 0.33032, 0.10219, 0.17489, 0.26515, 0.31199, 0.063898, 0.1182, 0.19703, 0.26871, 0.045055, 0.082478, 0.14751, 0.21937, 0.034767, 0.060288, 0.10815, 0.18865, 0.017343, 0.023308, 0.030312, 0.080942 0.010988, 0.010027, 0.012595, 0.013208, 0.0044378, 0.0050367, 0.007641, 0.0075742, 0.0031212, 0.005471, 0.0023857, 0.0096344, 0.004087, 0.0019016, 0.006205, 0.020668, 0.0025152, 0.0020285, 0.0039214, 0.012822, 0.00043641, 0.00055117, 0.0010622, 0.003141, 0.00020276, 0.00023033, 0.0010985, 0.0010405

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.76419, 0.45666, 0.25652, 0.16829, 0.11854, 0.093686, 0.054054 .032442, .013252, .012617, .0085441, .005527, .0017459, .00062193 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.76419, 0.80169, 0.82451, 0.85141, 0.45666, 0.68378, 0.8154, 0.86986, 0.25652, 0.43459, 0.66544, 0.83262, 0.16829, 0.29358, 0.48126, 0.69943, 0.11854, 0.20404, 0.34253, 0.59125, 0.093686, 0.14943, 0.25307, 0.51915, 0.054054, 0.067506, 0.085897, 0.21106 0.032442, 0.043917, 0.042233, 0.0095133, 0.013252, 0.0084313, 0.023269, 0.01513, 0.012617, 0.0056627, 0.0086734, 0.017897, 0.0085441, 0.003882, 0.0078432, 0.052522, 0.005527, 0.0025057, 0.0039495, 0.039797, 0.0017459, 0.0017848, 0.0035247, 0.011518, 0.00062193, 0.0012843, 0.0010686, 0.002401

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.55751, 0.39467, 0.26512, 0.17411, 0.12033, 0.086001, 0.036371 .0075946, .005595, .0042456, .00568, .0048842, .002313, .00049314 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.55751, 0.56255, 0.56785, 0.58454, 0.39467, 0.4926, 0.56662, 0.562, 0.26512, 0.37128, 0.50313, 0.57582, 0.17411, 0.27137, 0.39351, 0.53095, 0.12033, 0.20031, 0.30487, 0.46169, 0.086001, 0.14549, 0.23471, 0.39465, 0.036371, 0.058668, 0.095081, 0.21265 0.0075946, 0.017581, 0.014201, 0.005585, 0.005595, 0.0047018, 0.012246, 0.022603, 0.0042456, 0.0035865, 0.0081421, 0.0054892, 0.00568, 0.0027604, 0.0041534, 0.013642, 0.0048842, 0.0017498, 0.0031374, 0.016814, 0.002313, 0.0013403, 0.0029052, 0.003605, 0.00049314, 0.00084767, 0.00089771, 0.0015101

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.85845, 0.62076, 0.43628, 0.30625, 0.24044, 0.19269, 0.10768 .0.0224439, 0.0111706, 0.0095075, 0.0129382, 0.0093905, 0.0026913, 0.0024300 0, 1, 2.5, 5 0.00000, 0.0014, 0.0028, 0.0042, 0.0056, .0070, .0140 0.85845, 0.90713, 0.94177, 0.95054, 0.62076, 0.83675, 0.93042, 0.92862, 0.43628, 0.67716, 0.87094, 0.94269, 0.30625, 0.51206, 0.77736, 0.88470, 0.24044, 0.40324, 0.60534, 0.82591, 0.19269, 0.31550, 0.49199, 0.78601, 0.10768, 0.17867, 0.24798, 0.50439 0.0224439, 0.0223095, 0.0293059, 0.0159658, 0.0111706, 0.0105817, 0.0186839, 0.0148995, 0.0095075, 0.0107458, 0.0224771, 0.0174710, 0.0129382, 0.0081189, 0.0091454, 0.0513437, 0.0093905, 0.0053745, 0.0250836, 0.0373250, 0.0026913, 0.0038961, 0.0073887, 0.0121860, 0.0024300, 0.0021880, 0.0027920, 0.0059692

This widget displays the data we collected for our LacI mutants. You can choose any combination of mutants to display together by toggling them on or off with the buttons on the right side. This allows you to easily view the strength of the mutant of interest compared to wildtype or any other mutant. You may also wish to view our data in 3D which can be done by clicking the 3D button. This will present you with a graph of the relative fluorescence for the promoter of interest plotted at varying IPTG and arabinose levels. You can drag the 3D plot with your mouse to view the data from different angles and as with the 2D graphs, you can toggle any combination of promoters you wish. When #3 and #6 are plotted together, their planes intersect suggesting that the binding affinity and transcriptional rate differ.

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