Reporter: Week 13 August 9-13

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Contents

Tuesday, August 9

Clone GusA and LacZα

Wednesday, August 10

Clone GusA and LacZα

Imp linker+XylE

Colony PCR clones from August 9

Results: failure

Thursday, August 11

Colony PCR August 10 clones

Results: LacZα worked, all else failed

tev protease mutagenesis PCR reaction, following Richard Lab double primer site directed mutagenesis

Friday, August 12

Clone GusA into K3

tev protease mutagenesis

Sequencing results from 8/10: failure (part of LacZα missing inexplicably

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