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Reporter: Week 13 August 9-13
From 2011.igem.org
Contents |
Tuesday, August 9
Clone GusA and LacZα
Wednesday, August 10
Clone GusA and LacZα
Imp linker+XylE
Colony PCR clones from August 9
Results: failure
Thursday, August 11
Colony PCR August 10 clones
Results: LacZα worked, all else failed
tev protease mutagenesis PCR reaction, following Richard Lab double primer site directed mutagenesis
Friday, August 12
Clone GusA into K3
tev protease mutagenesis
Sequencing results from 8/10: failure (part of LacZα missing inexplicably
