Reporter: Week 11 July 26-30

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Sunday, July 24

No colonies grew for Imp linker+KylE

Colony PCR small linker+XylE

Clone LacZα and GusA into K3 with new primers

Monday, July 25

tev protease mutagenesis

clone GusA and LacZα into K3

Tuesday, July 26

Place linkers in front of XylE

Colony PCR GusA and LacZα clones

Results: looked good on gel, grown up in culture

Wednesday, July 27

Miniprep and sequence GusA and LacZα clones

Results: Failure

Thursday, July 28

Clone GusA and LacZα into K3

Results from 7/22 assemblies:

Only Pr-RBS-XylE-small linker-tev cleavage site-GFP worked

Friday, July 29

Pr-RBS-XylE-linkers+cleavage sites+GFP

Colony PCR of GusA and LacZα clones

Results: failure

Saturday, July 30

Colony PCR of 7/29 assemblies

Results: failure

Back to Wetlab

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