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Reporter: Week 11 July 26-30
From 2011.igem.org
Contents |
Sunday, July 24
No colonies grew for Imp linker+KylE
Colony PCR small linker+XylE
Clone LacZα and GusA into K3 with new primers
Monday, July 25
tev protease mutagenesis
clone GusA and LacZα into K3
Tuesday, July 26
Place linkers in front of XylE
Colony PCR GusA and LacZα clones
Results: looked good on gel, grown up in culture
Wednesday, July 27
Miniprep and sequence GusA and LacZα clones
Results: Failure
Thursday, July 28
Clone GusA and LacZα into K3
Results from 7/22 assemblies:
Only Pr-RBS-XylE-small linker-tev cleavage site-GFP worked
Friday, July 29
Pr-RBS-XylE-linkers+cleavage sites+GFP
Colony PCR of GusA and LacZα clones
Results: failure
Saturday, July 30
Colony PCR of 7/29 assemblies
Results: failure
