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Week 2
| 7/16/2011 |
All but negative control grew 12.2.13 is slightly pink probably due to the constant red
Glycerol stock- 350 µL of cells and 150 µL if 50% glyrcerol
Miniprep and transformation results- the plate had about 50 to 60 colonies
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7/17/2011
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Incubate in 3 mL LB broth overnight at 37 degrees Celsius and 2020 rpm
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7/18/2011
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Colony grew, negative control didn’t
Transfomation- 50 µL DH5a, 2 µL DNA at 37 degrees Celsius overnight (all carb)
Glycerol stock- 350 µL of cells and 150 µL of 50% glycerol
Miniprep
Dissolving and diluting primers (10 pmol/µL)
PCR and gel electrophoresis and purification
Digestion for 1 hour and 30 minutes at 37 degrees Celsius
Gel Electrophoresis and Purification
Digestion and ligation
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7/19/2011
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Transformation- 50 µL DH5a, 2 µL DNA at 37 degrees Celsius overnight |
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