| 9/11/11 | Incubation: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
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| | |
| 9/12/11 | Glycerol Stock: ToxR-FGFR, ctx-CheZ*, ctx-CheZ, ToxR, FGFR, CheY, CheZ,
Test Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Full Digestion/Gel Electrophoresis: FGFR-ToxR, ctx-CheZ-RBS-GFP
Transformation: ToxR'MalE + ctx-CheZ-RBS-GFP, ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP, ToxR'MalE/Zip L267S + ctx-CheZ-RBS-GFP
Plating: ToxR'MalE/ZIP + ctx-CheZ-RBS-GFP
Ligation: scp, Pyear, TetR, ToxR-FGFR, ctx
Incubation: CheZ* (Chlora)
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| | |
| 9/13/11 | Transform of PyeaR-ToxR-FGFR, TetR-ToxR-FGFR, scp-ToxR-FGFR
Full Digestion: ToxR-FGFR (at E/X as vector)
Ligation: Vector-> ToxR-FGFR Insert-> ctx
Incubation: CheY
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| | |
| 9/14/11 | Transformation: ctx-ToxR-FGFR
Test Digestion: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/Zip L267S
Incubation: scp-ToxR-FGFR, TetR-ToxR-FGFR, PyeaR-ToxR-FGFR (Carb)
Miniprep: CheY
PCR: CheY
Incubation: ToxR'MalE, ToxR'MalE/ZIP, CheZ*
|
| | |
| 9/15/11 | PCR: CheY
Full Digestion: CheY (X/S), vector pSB1C3
Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP
Test Digestion: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP
Dephosphorylation: Vector pSB1C3
Ligation: CheY + pSB1c3
Full Digestion/Gel Elctrophoresis/Purification: pYeaR-ToxR-FGFR, scp-ToxR-FGFR, ToxR'MalE, ToxR'MalE/ZIP, CheZ, CheZ*, ctx-CheZ-RBS-GFP, ctx
Ligation: ctx + CheZ*
Transformation: pSB1C3 Vector, pYeaR-ToxR-FGFR, ctx-CheZ-RBS-GFP, CheZ, ctx-GFP, ctx, Chey
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| | |
| 9/16/11 | Digestion: ctx-CheZ
Incubation: ToxR'MalE/ZIP, ToxR'MalE,
Ligation: ctx + CheY (retry)
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| | |
| 9/17/11 | Incubation: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY |
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