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9/18/11 | Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY
Test Digestion: pYeaR-ToxR-FGFR, ctx-CheZ*, CheY
Colony PCR: pYeaR-ToxR-FGFR
Test Digestion: CheY (X/S)
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9/19/11 | Full Digestion/Electrophoresis: CheY, CheZ*, pSB1C3
PCR/Gel Electrophoresis: CheY
Ligation:
ctx + CheZ*, ctx + CheY, pYeaR-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3
Incubation:
scp-ToxR-FGFR, ToxR'MalE/ZIP, ToxR'MalE
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9/20/11 | PCR/Gel Purify: ctx
Maltose Induction Experiment: DATA COLLECTION
Miniprep: scp-ToxR-FGFR
Full Digestion/Gel Purification: CheZ*, CheY
Ligation: ctx + CheZ*, ctx + CheY, ctx + CheZ-RBS-GFP
Transformation: ToxR'MalE + ctx-GFP, ToxR'MalE/ZIP + ctx-GFP, ToxR'MalE/ZIP L267S + ctx-GFP
Icubation: pYeaR-ToxR-FGFR (Chlora)
Miniprep/Glycerol Stock: pYeaR-ToxR-FGFR
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9/21/11 | PCR: ctx (retry)
Test Digestion/Purification: pYeaR-ToxR-FGFR
Full Digestion/PurificationL pYeaR-ToxR-FGFR
Dephosphorylation: pSB1C3
Incubation: ctx-gfp, BL21 E. coli
Transformation: Germany Parts (FHK12)
Gel Purification: ctx
Ligation: ctx + CheY
Incubation: ctx-CheZ*
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9/22/11 | Miniprep/Glycerol Stock: ctx-GFP, ctx-CheZ, ctx-CheY
Full Digestion: pSB1C3
Make BL21 competent
Dephosphorylation: pSB1C3
Ligation: ctx + CheY, ctx + FGFR-ToxR
Transformation: (BL21) pcst-RBS-LuxI, AHL inducible colicin, ctx-CheY, ctx-ToxR-FGFR
Ligation: scp-ToxR-GFP + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, ctx-Chez + pSB1C3
Incubation: psct-RBS-LuxI-term
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9/23/11 | Miniprep: psct-RBS-LuxI-term
Dissolving/Diluting Primers: Germany Parts
1:20 Dilution of scp-ToxR-FGFR + ctx-GFP, pYeaR-ToxR-FGFR + ctx-GFP
Glycerol Stock: scp-ToxR-FGFR + ctx-GFP, pYeaR-ToxR-FGFR + ctx-GFP
Transformation: German Parts
Incubation: scp-ToxR-FGFR, pYeaR-ToxR-FGFR
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9/24/11 | Dilution 1:10 for Optical Density readings: scp-ToxR-FGFR, pYeaR-ToxR-FGFR
Growing from glycerol stock (backup): scp-ToxR-FGFR, pYeaR-ToxR-FGFR
PCR: German Parts
Digestion: pSB1C3 (X/S)
Heparin/FGF Induction Experiment: DATA COLLECTION (Fluorescence)
Dephosphorylation: Germany Parts, pSB1C3
Ligation:
ctx-CheZ + pSB1C3, scp-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, pSB1C3-CheY + ctx, pSB1C3-CheZ* + ctx, pSb1C3-ToxR-FGFR + ctx
Transformation:
ctx-CheZ + pSB1C3, scp-ToxR-FGFR + pSB1C3, ctx-CheZ-RBS-GFP + pSB1C3, pSB1C3-CheY + ctx, pSB1C3-CheZ* + ctx, pSb1C3-ToxR-FGFR + ctx
Transformation:
ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S |
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9/25/11 | Dilution 1:20 for experimentation: ToxR'MalE, ToxR'MalE/ZIP, ToxR'MalE/ZIP L267S
Running Maltose Induction Experiment: DATA COLLECTION (fluorescence)
Incubation: scp-ToxR-FGFR, pYeaR-ToxR-FGFR,
Incubation of fluorescent parts: scp-ToxR-FGFR, pYeaR-ToxR-FGFR,
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9/26/11 | Dilution 1:5 for experimentation: pYear-ToxR-FGFR, scp-ToxR-FGFR
Heparin/FGF Induction Experiment (re-run): DATA COLLECTION (fluorescence)
Incubation (for iGem Registry): FGFR, CheZ*, ToxR-FGFR |
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